Our working group uses numerous biochemical and molecular biological methods. We also have many years of experience with specialised plant research methods, such as the laser aphid technique for extracting phloem sap and non-aqueous fractionation for determining subcellular metabolite concentrations.
Metabolite analyses
- Mono-, di- and oligosaccharides (HPLC, amperometric detection)
- Amino acids and other nitrogen compounds (HPLC, fluorescence detection)
- Inorganic anions and cations (HPLC, conductivity detection)
Molecular biological methods
- RNA isolation from plant material including wood
- Quantitative RT-PCR
- In situ hybridisation of RNA
Phloem sap extraction
The special laser aphid technique is used to extract pure phloem sap from intact plants
Determination of subcellular metabolite concentrations
- Non-aqueous fractionation for the separation of subcellular components
- Determination of subcellular volumes
- Determination of subcellular metabolite concentrations
